3rd Generation ELISA
Hi,
I am male, 23 years old and I had unprotected sex and i did the folowing tests:
- 16 days post exposure: RNA PCR and ELISA
- 30 days post exposure: RNA PCR, ELISA and WESTERN BLOT
- 80 days post exposure: ELISA and WESTERN BLOT
All my results are negative. With 75 days post exposure I had symptoms like high fever and severe diarrhea for 2 days. Also the night after my fever has gone, I had sweats for the whole night.
Also i have thyroid problems, i take syntroid every day since i was 8 years old.
I'm worried if i need to re-test, are my results conclusive, is possible that those symptoms are refered to the accute infection of HIV. Can my thyroid problem affect my window period?
Thanks,
David
Hello,
I slept with a person of unknown HIV status 5 months ago... I feel it was a really big mistake... and I would never do it again... we had unprotected sex...
I got tested at 4 months (INSTI) and now (5 months) with ELISA test. Both came negative. The doctors told me the accurancy is almost 100% and I need no other worries about it. There are internet sources which say you need 6 months to get conclusive results tho. I am so sad about it... I think I cant wait another month... of suffering... :( my tears are so real now... i did a mistake... but it seems it never will get a definitive end.
Thanx for your answer.
Yours William.
I am a male and I was recently given oral by a male a few times and then later he told me that he's hiv positive undetectable. I am worried because, I ejaculated in his mouth more than two times and kissed him. Is it possible for me to have gotten hiv from him? how long should I wait before I can get tested for hiv? also is it ok to have unprotected oral and, anal with a condom on, with someone that's hiv positive undetectable? will I contract hiv this way?
I would just like to know why the PCR RNA test isn't considered a conclusive test according to the FDA? I've read about many different window periods, some as early as 3 days, and others as early as 14 days. They seem pretty reliable from what I've read, the only problems that seem to be in the way is the cost, and the "high" false positive chances, as high as 2.5%. Which isn't a concern for someone who has gotten a negative because the false negative is even lower than a false positive, I'm guessing under the 1% range, so about a 99% percent accuracy. Which isn't much difference from a Gold Standard ELISA test post 3 months, which is around 99.7%.
Are there any statistically significant numbers that point that a negative PCR test turns into a positive ELISA post 3 months?
Sorry for asking so many questions, but it's just interesting to me why an RNA PCR test isn't considered on the same level of reliability as an ELISA.
They don't seem to have a significant difference in accuracy for one to be FDA approved and the other not to be. PCR tests have been used for a while now, and it just seems odd to me why they aren't considered reliable enough for diagnostic purposes.
I think a much shorter window period would save alot of people of that worry for the 3 month period. The expense is definitely a concern, but for those who have access to this test, it doesn't seem like a 3 month ELISA test is necessary unless for anything than re assurance.
My last sexual exposure (Dec 15th) was with someone whose status i don't know. I tested at 8 weeks with a lab based EIA, 14 weeks with a Oraquick swab test, and 16 weeks (Apr 5th ) with Oraquick swab test. All came back negative. Do I need to test out till 6 months or am I conclusively HIV negative?
(my last exposure was receiving oral sex and mutual masturbation with a female and I haven't had any risky behavior since then)
hi sir i am male from india, i had sexual high risk exposure with sex worker and she was hiv positive after i did pcr proviral dna pcr test after 50 days the result was non reactive then after 6 weeks rapid test for hiv1/2 (SD bioline 3.0) the result was negative then after 3 months elisa result was non reactive then after 5 months elisa result was negative then after 6 months 4th generation elisa test the result was also non reactivethen after 8 months westernblot test that was also negative, then after 8 months rna pcr viral load test for hiv 1 the result was <20 means not detected but sir my cd4 cell is 453 and cd8 177 and cd4/cd8 ratio high 2.34, then after 9 months tested by two rapid tests kits result was negative ,now i did 3rd generation hiv rapid test after 10 months the result is negative then after 10,11 and now 12 months sd bioline3.0 rapid test all were negative and now after 13 months i did duo test from dr lal path lab the result is negative now after 15 months i have diagnose with jaundice my hcv and hbv antibodies tests negative after 15 months i have some ques regarding this:
1) is hiv 4th generation test conclusive after 13 months?
2) is any case who takes more than 1 year to show positive result?
3) i have white coated tounge from 15 months and pain on it is this cause by hiv/ hcv/hbv?
4) i have 2 kg weight loss is this due to hiv?
5) why my cd4 and cd8 low and cd4/cd8 ratio high?
6) is hiv 4th generation test detects all sub type?
7) is 13 months long enough for hiv testing or i have to wait more?
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