Join our mailinglist

Please leave this field empty

AIDS Vancouver

Prevent. Act. Support.

PCR RNA & DNA NAAT Tests

Monday, May 14, 2012
Question:

 

Doctor i did mistake of doing unprotected sex with prostitude
I did combo on 98 th day postexposure
I did pcr dna and combo on 123 rd day post exposure
tests were negative
Doctor I read that not all the subtypes of hiv1 and 2 are detected by the hiv tests
my questions are
1. Is it true?
2. In my case as my symptoms are still persisting after 4 months , am i affected from any of that subtype?
3. How long it take to come pcr dna results back after giving sample for testing?In my case I was submitted the result very early that is 2 days after but before giving the blood sample the patholgist told me that resuly will come after an week.
4.Is 36 hours time enough for the pcr results to come ?
5. i did Combo test again on 123 rd day post exposure but the index was(0.11) and last time it was (0.08) ..What is this increase in value about? I am scared of this..
6.Do Hepatatis virus(no medications) extend the window period of hiv?( i dont know whether i have hepatitis)




Sunday, May 13, 2012
Question:

Hi,
I am male, 23 years old and I had unprotected sex and i did the folowing tests:

  • 16 days post exposure: RNA PCR and ELISA
  • 30 days post exposure: RNA PCR, ELISA and WESTERN BLOT
  • 80 days post exposure: ELISA and WESTERN BLOT

 

All my results are negative. With 75 days post exposure I had symptoms like high fever and severe diarrhea for 2 days. Also the night after my fever has gone, I had sweats for the whole night.

Also i have thyroid problems, i take syntroid every day since i was 8 years old.

I'm worried if i need to re-test, are my results conclusive, is possible that those symptoms are refered to the accute infection of HIV. Can my thyroid problem affect my window period?

Thanks,
David





MJ

Tuesday, May 8, 2012
Question:

 

Hello-thanks for such a great site
how reliable is PCR after finishing PEP?
 




HIV

Monday, April 30, 2012
Question:

 

After my exposure 21 days i had a pcr test which came back negative and then after the exposure 2 months i had antibodies test which came also negative
but i had symptoms like diarrhea, fatigue, burning skin ,sore throat,night sweating muscle pain , joint pain ,headache and fever . even thu now i become week i lost weight ,still my body skin have burning sentation and sore throat which dont want to go. 




Monday, April 30, 2012
Question:

I would just like to know why the PCR RNA test isn't considered a conclusive test according to the FDA? I've read about many different window periods, some as early as 3 days, and others as early as 14 days. They seem pretty reliable from what I've read, the only problems that seem to be in the way is the cost, and the "high" false positive chances, as high as 2.5%. Which isn't a concern for someone who has gotten a negative because the false negative is even lower than a false positive, I'm guessing under the 1% range, so about a 99% percent accuracy. Which isn't much difference from a Gold Standard ELISA test post 3 months, which is around 99.7%.

Are there any statistically significant numbers that point that a negative PCR test turns into a positive ELISA post 3 months?

Sorry for asking so many questions, but it's just interesting to me why an RNA PCR test isn't considered on the same level of reliability as an ELISA.

They don't seem to have a significant difference in accuracy for one to be FDA approved and the other not to be. PCR tests have been used for a while now, and it just seems odd to me why they aren't considered reliable enough for diagnostic purposes.

I think a much shorter window period would save alot of people of that worry for the 3 month period. The expense is definitely a concern, but for those who have access to this test, it doesn't seem like a 3 month ELISA test is necessary unless for anything than re assurance.

 





Wednesday, April 25, 2012
Question:

I had a protected sex with a sex worker, i´m sure the condom did not fail, but it last 30 minutes. 

 I´m worried of getting HIV.

So  for peace of my mind I performed a PCR VIRAL LOAD TEST THAT COST ME 350$ AT 20 DAYS AND 27 DAYS POST EXPOSURE THAT CAME NOT DETECTABLE AND A DUO TEST 30 DAYS AFTER AND CAME NEGATIVE.

I REMEMBER SHE TOLD ME THAT SHE  GOT TESTED 10 DAYS LATER  FOR HIV AND ALL STI AND CAME NEGATIVE . I DON'T HAVE ANY SYMPTOMS SO FAR. SHOULD I WORRY?

I also get tested to Syphillis and came negative, I dont know why I put myself in this situation. please help-me. 





Tuesday, April 24, 2012
Question:

hi sir i am male from india, i had sexual high risk exposure with sex worker and she was hiv positive  after i did pcr proviral dna pcr test after 50 days the result was non reactive then after 6 weeks rapid test  for hiv1/2 (SD bioline 3.0) the result was negative then after 3 months elisa result was non reactive then after 5 months elisa result was negative then after 6 months  4th generation elisa test the result was also non reactivethen after 8 months westernblot test that was also negative, then after 8 months rna pcr viral load test for hiv 1 the result was <20 means not detected  but sir my cd4 cell is 453 and cd8 177 and cd4/cd8 ratio high 2.34, then after 9 months tested by two rapid tests kits result was negative ,now i did 3rd generation hiv rapid test after 10 months the result is negative then after 10,11 and now 12 months sd bioline3.0 rapid test all were negative and now after 13 months i did duo test from dr lal path lab the result is negative now after 15 months i have diagnose with  jaundice my hcv and hbv antibodies tests negative after 15 months  i have some ques regarding this:

1) is hiv 4th generation test conclusive after 13 months?
2) is any case who takes more than 1 year to show positive result?
3) i have white coated tounge from 15 months and pain on it is this cause by hiv/ hcv/hbv?
4) i have 2 kg weight loss is this due to hiv?
5) why my cd4 and cd8 low and cd4/cd8 ratio high?
6) is hiv 4th generation test detects all sub type?
7) is 13 months long enough for hiv testing or i have to wait more?
 





Tuesday, April 24, 2012
Question:

Hi, I had sex with a sex worker on February 17th 2012 in the Red Light District. I was ignorant of proper condom usage and put on two condoms, she gave me oral for about 3 minutes, then I had vaginal sex with her for about 1 minute and a half, pulled out because it was my first time with a sex worker and it felt kind of wrong. She then pulled off my condoms, which didn't look ripped but intact, and put a new one on finishing me with a hand job.

I started sweating that very night from fear, and haven't had a good night sleep without a nightmare since (67 days now) pain in my body prevents me as well. I began developing joint pain, then muscle burning on my shoulders, and hips 2 weeks later that migrated from my feet up to my shoulders. 13 days after Amsterdam I got a PCR test, my doctor told me any virus would show up in 8 days. It came out negative as well as all STDs. I know it was too short for the STDs to show up in a test. I don't have any ulcers, no vomiting, no swollen lymph nodes, no rashes, no mysterious fevers. Just pain throughout my body that migrates, I don't know what to do. I checked for lyme disease, rhuematoid arthritis, and all came out clear. 
 
I'm still left with the real pain in my body, it's been 7 weeks of pain in my body, and I don't know if it's acute HIV because it usually doesn't just last for 7 weeks from what I've read. There is a part of me that I have HIV, it shows up in my nightmares, so I'm gonna put a rest on the 90 day mark, but do you know if anxiety/ depression can cause this? Or another STIs that I should test for associated with migrating pain? Thanks again, and I'm on my 68th day.




Monday, April 16, 2012
Question:

 

Sir 100 days ago I had unprotected sex with a female sex worker... 30 days after that incident i suffered from diarrehea badly approx for 15 days and after that mild nausea , headache,tiredness(lack of energy), abdominal pains for 2 months.. Sir I did following tests->
  1. Western Blot->19th day->neg
  2. PCR DNA    -> 36th day->neg
  3. Tridot(J. Mitra)->92nd day->neg
  4. Combo test(p24 antigen,hiv1 ab,hiv2 ab)->98th day->neg

Sir please let me know what i should do now...

I have suffered very badly from all this.. I am in huge tension..Are my test results conclusive?? or whether I should do any other test??? plz reply ... 




Friday, April 13, 2012
Question:

 

Sir 100 days ago I had unprotected sex with a female sex worker... 30 days after that incident i suffered from diarrehea badly approx for 15 days and after that mild nausea , headache,tiredness(lack of energy), abdominal pains for 2 months.. Sir I did following tests-> Western Blot->19th day->neg

PCR DNA    -> 36th day->neg

Tridot(J. Mitra)->92nd day->neg

Combo test(p24 antigen,hiv1 ab,hiv2 ab)->98th day

->neg

Sir please let me know what i should do now...

I have suffered very badly from all this.. I am in huge tension..Are my test results conclusive?? or whether I should do any other test??? plz reply ...





Pages

Subscribe to PCR RNA &amp;amp; DNA NAAT Tests