ELISA 4th gen test accuracy

Originally Posted: 
Friday, June 10, 2011
Question: 

on the 12/5 im sure that a started having vaginal intercourse with a man, but i didnt check whether or not he was still with the condom as we changed position. so, we have spoon position and he took his penis out of me and few seconds i turned to him he was masturbating without codom. he told me that he intercoused with condom but because he couldnt ejaculte, he decide to masturbate. i touch his penis e kissed it to and i couldnt feel neither my fluid taste nor a creamy, sticky sentation in my hands.
i got tested for p24 and fourth generation 12 days after exposure and came back negative. are these tests accurate after 12 days? i also have the p24 and fourth generatio book again for the 28/06 (47 afert exposure)are these time enough to get an accurate result? i also have pcr to book for the 24/06 (6 weeks after exposure.
how accurate are these tests? how accurate were the first time i get tested? i havnet felt any symptom.
but i cant sleep with anxiety, please help me, thanks.

Answer: 

Hello and thank you for using AIDS Vancouver as your source for HIV-related information.
 
The P24 antigen test alone can detect HIV infection only within the first couple weeks of exposure. So your P24 test at 12 days is a good indication that you are HIV negative.
 
The fourth generation EIA or DUO test looks for both HIV antibodies and the P24 protein and can detect 95% of infections by 6 weeks after exposure. This is the newest type of HIV test and it can detect very small amounts of antibodies and P24 proteins with a 99.9% accuracy. Current guidelines suggest that you retest at 3 months for conclusive results so I would recommend that you do this to reconfirm your negative result.
 
I hope this helps you.
 
All the Best,
Carlo
Helpline Volunteer

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